BLAST Guide

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BLAST
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PSI-BLAST
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This step-by-step BLAST guide covers the basic strategy for setting up a protein-based BLAST search, describes the format and significance of the search output and discusses the use of PSI-BLAST and other post-BLAST tools to extend and enhance the results of a BLAST search.

*Setting up a BLAST search*
	Step 1. Plan the search Step 2. Enter the query sequence Step 3. Choose the appropriate search parameters Step 4. Submit the query
*Deciphering the BLAST output*
	Step 1. Examine the alignment scores and statistics Step 2. Examine the alignments Step 3. Review search details to plan the next step
*Post-BLAST analysis*
	Perform a PSI-BLAST analysis Create a multiple alignment Try motif searching with PHI-BLAST

For a more interactive introduction to the same material try the BLAST tutorial.

Revised May 2, 2000

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