| Multiple sequence alignment of MJ0577 and 6 High-Scoring BLAST Hits |
This multiple alignment was constructed within BLAST. Five of the top hits from the BLAST analysis were aligned. The query sequence is shown at the top with the designation "blast_tmp". Identities are show as dots (instead of letters). The top hit is MJ0577 itself and is therefore completely represented as dots. Columns of dots represent residues that are identical in all 6 sequences. Conserved residues can be spotted by inspection. Unaligned residues are shown as dashes within the sequence line.
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Sequences represented in the alignment:
sp|Q57997|Y577_METJA PROTEIN MJ0577 >gi|2128018|pir||A64372... 314 3e-85
pir||B71132 hypothetical protein PH0823 - Pyrococcus horiko... 107 7e-23
sp|Q57951|Y531_METJA HYPOTHETICAL PROTEIN MJ0531 >gi|212801... 91 4e-18
pir||A69233 conserved hypothetical protein MTH993 - Methano... 85 4e-16
pir||A69220 conserved hypothetical protein MTH898 - Methano... 81 5e-15
pir||A69071 conserved hypothetical protein MTH153 - Methano... 80 8e-15
pir||D69008 conserved hypothetical protein MTH1065 - Methan... 79 2e-14 |
The Alignment:
blast_tmp 1 MSVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVA 60
Q57997 1 ............................................................ 60
B71132 1 .IF.FR.V.F......G.YR.VEVFEKRNKMEVG..........GTLEE-----.MD.YS 55
Q57951 25 L....VI...G.DVSLE.A..AINIAKEFDA..YAIY.V.---------V.PFV.LP 72
A69233 1 ..S...L...G.KQ.NK.AE.AIWIARESGA.I.A.T.MET---------.S.V.LP 48
A69220 1 ..R...LA..G..CSMQ.AGYAIETAAQNRA.LLA.T.TETYPLDN-------.P.E 50
A69071 1 .FE..MV...G..Y.AR.EDMAIELAGRLGSV..AV.....KL.YPF.V-------- 49
D69008 1 ..RR..I...G.GD.RK.TR.AFHIAGMSGADILAIS.V.TSYR.IW.--------- 48
blast_tmp 61 GLNKSVEEFENELKNKLTEEAKNKMENIKKEL---EDV--GF----KVKDIIVV--GIPH 109
Q57997 61 ................................---...--..----........--.... 109
B71132 56 FFYDNA.IELKDI.E..K...SR.LQEKAE.V---KRAFRAK----N.RT..RF--...W 106
Q57951 73 A--EGSW.LIS..---.K..GQEALKKV..MA---.EW--.V----.IHTEMLE--.V.A 116
A69233 49 A-----DDLIIR.REM.E...SRSL.AV..LV---.ES--.A----DI.LTVRTDE.S.A 94
A69220 51 E.TRK.----T..FR.ES...LQ.V.DLAVS.----.T--PV----..RKMM.D--.S.A 94
A69071 50 ----------------.ED.G.EILASVQRKG---REA--.V----Q.DEVL.F--.S.A 82
D69008 49 ------.DISRR.EEI.KKQ.EKAISIL.E.FSSQQEL--.HMTETRLDTV.LE--.N.A 98
blast_tmp 110 EEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS 162
Q57997 110 ..................................................... 162
B71132 107 D....V..E.N.SL..LP.R..LS.SHEF...TVMR.LR.TK....II. 155
Q57951 117 N...EF..KKKA.L.V..TT...G.ER......A.R...NAHC......K 166
A69233 95 .A.LRTV.K....LVV..TS..HG.DRF.....A.K.VRSAGC..... 142
A69220 95 .T.L.V.DE.N..L.VV.AS..HA.ERF.....S.KIVRHARV.....HS. 145
A69071 83 HDMK..T.KT.A.LVVIA...RSG.EKL.M...A.TTL.TVDV...L.. 131
D69008 99 .V.LEVM...D..LVV...S..HG.DR.IS..I.RK.L.SAT..MM.. 146
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